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Stellaris RNA FISH洗涤缓冲液B:Stellaris® RNA FISH Wash Buffer B 20 mL

价格:¥

货号:SMF-WB1-20
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

洗涤缓冲液B(20 mL):足够做50-100个样本,次使用前须添加无核酶水。 Wash Buffer B (20 mL): Enough provided for 50-100 samples. Nuclease-free water must be added upon first use. Stellaris RNA荧光原位杂交缓冲液包含的添加剂,能够增强Stellaris RNA荧光原位杂交探针的检测信号,尤其适合于一些典型的荧光背景高的应用。 Stellaris® RNA FISH Buffers contain proprietary additives to enhance detection with Stellaris RNA FISH probe sets, particularly for applications that typically exhibit more pronounced background fluorescence. Before getting started with your first experiments, please download our Getting Started Guide. You can also learn more about the Stellaris RNA FISH technology by downloading our white paper or by visiting our RNA FISH applications page. Furthermore, The BiosearchTech Blog contains the most up-to-date tips and tricks for running Stellaris RNA FISH experiments.

Stellaris原位杂交探针,FAM标记的自定义设计探针:Stellaris® FISH Probes, Custom Assay with FAM 5 nmol

价格:¥

货号:SMF-1025-5
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

属性: 更大激发荧光(Lambda Max):495; 更大发射荧光:520; 产品用法: 溶解后的探针注意尽量避免反复冻融。当天或短期使用,探针溶液可以避光放在+2 ~ +8 °C储存一个月,超过1个月,推荐分装后将溶解后的探针放在-15至-30 °C避光保存。 Protocols:详细Protocol根据样本类型而异,请上以下网站下载:https://www.biosearchtech.com/stellarisprotocols; 储存及运输: 运输条件:常温/蓝冰/干冰; 储存条件:+2 ~ +8 °C; Properties: Product Usage: Absorption Maximum (Lambda Max): 647; Fluorescence Maximum: 670; Product Usage: Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols. Storage and Handling: Shipping Conditions: Ambient; Storage Conditions: +2 to +8 °C 设计您自己的自定义Stellaris®RNA FISH探针来检测你感兴趣的RNA。通过使用LGC Biosearch在线Stellaris RNA FISH探针设计工具Stellaris RNA FISH probe designer,可以设计得到多达48个荧光单独标记的寡核苷酸来结合并可视化待检测的靶标RNA。在开始您的Stellaris RNA FISH自定义探针设计之前,可以通过在DesignReady探针界面的搜索框内输入感兴趣的RNA名称来确认LGC Biosearch是否已经有针对您需要检测的靶标有设计好的探针。LGC Biosearch还提供Stellaris ShipReady探针可作为您实验的阳性对照,以及Stellaris缓冲液,作为探针的完美搭配,能够让RNA的检测与分析更强大。 产品形式:一组Stellaris FISH探针集包含荧光标记的高达48种寡核苷酸的混合物,提供冻干粉形式。 交付量:5 nmol汇集的寡核苷酸(根据每种靶标更优的稀释度可以做200-400次杂交实验)。 价格:所有自定义FISH探针价格统一。 设计周期:5-7个工作日。 Design your own Custom Stellaris® RNA FISH Probe Set to detect your RNA of interest. By using our Stellaris RNA FISH probe designer, you can design up to 48 individually fluorescently labeled oligonucleotides to bind along and visualize your target RNA. Before you begin designing your Stellaris RNA FISH probe set, check to see if we have already designed a set against your target of interest by entering your target into the search box on our DesignReady page. We also offer Stellaris ShipReady probe sets to be used as controls for your experiment and Stellaris Buffers, which are the perfect accompaniment to your probe sets, allowing for even more robust RNA detection and analysis. Product information: One set of Stellaris FISH probes contain a blend of up to 48 oligos labeled with a fluorophore. Probes arrive lyophilized and ready to use. Delivered Amount: 5 nmol of pooled oligos (200-400 hybridization experiments depending on optimal working dilutions for each target). Price: Same price for all custom Stellaris probes. Turnaround time: 5 to 7 business days.

Stellaris原位杂交探针,Quasar® 670标记的猫GAPDH探针:Stellaris® FISH Probes, Feline GAPDH with Quasar® 670 Dye 5 nmol

价格:¥

货号:VSMF-9039-5
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

属性: 更大激发荧光(Lambda Max):647; 更大发射荧光:670; 产品用法: 溶解后的探针注意尽量避免反复冻融。当天或短期使用,探针溶液可以避光放在+2 ~ +8 °C储存一个月,超过1个月,推荐分装后将溶解后的探针放在-15至-30 °C避光保存。 Protocols:详细Protocol根据样本类型而异,请上以下网站下载:https://www.biosearchtech.com/stellarisprotocols; 储存及运输: 运输条件:常温/蓝冰/干冰; 储存条件:+2 ~ +8 °C; Properties: Product Usage: Absorption Maximum (Lambda Max): 647; Fluorescence Maximum: 670; Product Usage: Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols. Storage and Handling: Shipping Conditions: Ambient; Storage Conditions: +2 to +8 °C 猫GAPDH探针包括一组Quasar® 670标记的寡核苷酸以一定的比例混合在一起,终浓度是5 nmol,按照标准条件操作能够做200-400次杂交。该探针用于利用荧光原位杂交的方法检测猫GAPDH的转录本。 探针设计依据:Product was designed against glyceraldehyde-3-phosphate dehydrogenase GAPDH a.k.a. (NCBI gene ID:493876) and nucleotides 1-1002 of NM_001003142.1. This is an inclusive probe set designed to detect the following variants: NM_001009307.1, and XM_006933438.2. 该探针没有在同物种或其它物种的同源和直系同源序列上验证可能的交叉杂交。

Stellaris原位杂交探针,CAL Fluor® Red 590标记的小鼠Egfr探针:Stellaris® FISH Probes, Mouse Egfr with CAL Fluor® Red 590 Dye 5 nmol

价格:¥

货号:VSMF-3000-5
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

属性: 更大激发荧光(Lambda Max):569; 更大发射荧光:591; 小鼠Egfr探针包括一组CAL Fluor® Red 590标记的寡核苷酸以一定的比例混合在一起,终浓度是5 nmol,按照标准条件操作能够做200-400次杂交。该探针用于利用荧光原位杂交的方法检测小鼠Egfr的转录本。 探针设计依据:Product was designed against epidermal growth factor receptor Egfr a.k.a. Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik (NCBI gene ID:13649) and nucleotides 1-2206 of NM_207655.2. This is an inclusive probe set designed to also detect the following variant: NM_007912 该探针没有在同物种或其它物种的同源和直系同源序列上验证可能的交叉杂交。 Stellaris® FISH Probes, Mouse Egfr with CAL Fluor® Red 590 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect Egfr transcripts in specimens using fluorescence in situ hybridization (FISH). Design Criteria: Product was designed against epidermal growth factor receptor Egfr a.k.a. Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik (NCBI gene ID:13649) and nucleotides 1-2206 of NM_207655.2. This is an inclusive probe set designed to also detect the following variant: NM_007912 The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Stellaris® FISH Probes, Custom Assay with ATTO 565 Dye, 5 nmol Total 1Unit

价格:¥

货号:SMF-0565-5
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 26 Bases, Salt-Free, 50 nmol 1Unit

价格:¥

货号:P1-5-26BASES-A
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 30 Bases, Salt-Free, 50 nmol 1Unit

价格:¥

货号:P1-5-30BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Custom Oligonucleotide, FLT3-20F-f, MB1638, 21 Bases, 5 FAM, RP HPLC purified, 200nmol 1Unit

价格:¥

货号:DNA-2-21BASES-A
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Base Charge, Standard Oligonucleotide Synthesis, 1 umol 1Unit

价格:¥

货号:COS-1
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Base Charge, Standard Oligonucleotide Synthesis, 50 nmol 1Unit

价格:¥

货号:COS-5
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2, 20 nmol del 1Unit

价格:¥

货号:DLO-Q6B2-2sp
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

ValuProbe?, 5' FAM/3' BHQ-1, provides 10 nmol pure 1Unit

价格:¥

货号:DLO-RFB-5-A
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Dual-labeled Probe, 5' FAM/3' TAMRA, 50nmol del 1Unit

价格:¥

货号:DLO-FT-1sp
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Dual-labeled Probe, 5' Quasar 670/Internal TBHQ-3/3' C3, 1 umol 1Unit

价格:¥

货号:DLO-Q6B3-1sp
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, 24 Bases each, Salt-Free, 1umol 1Unit

价格:¥

货号:P1-1-24BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 22 Bases, Salt-Free, 200 nmol 1Unit

价格:¥

货号:P1-2-22BASES-B
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 25 Bases, Salt-Free, 200 nmol 1Unit

价格:¥

货号:P1-2-25BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 22 Bases, Salt-Free, 1 umol 1Unit

价格:¥

货号:P1-1-22BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 18 Bases, Salt-Free, 200 nmol 1Unit

价格:¥

货号:P1-2-18BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 27 Bases, Salt-Free, 200 nmol 1Unit

价格:¥

货号:P1-2-27BASES-B
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 24 Bases, Salt-Free, 50 nmol 1Unit

价格:¥

货号:P1-5-24BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 27 Bases, Salt-Free, 200 nmol 1Unit

价格:¥

货号:P1-2-27BASES-A
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 16 Bases, 8 Internal Insoines, Salt-Free, 200 nmol del 1Unit

价格:¥

货号:P1-2sp-16BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 22 Bases, Salt-Free, 50 nmol del 1Unit

价格:¥

货号:P1-2sp-22BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 21 Bases, Salt-Free, 50nmol 1Unit

价格:¥

货号:P1-5-21BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 23 Bases, Salt-Free, 50 nmol 1Unit

价格:¥

货号:P1-5-23BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Primer, Avg. 26 Bases, Salt-Free, 50 nmol 1Unit

价格:¥

货号:P1-5-26BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

Base charge + Purification, Primer, Cartridge Purified, 200 nmol 1Unit

价格:¥

货号:P1C-2
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

定制寡核苷酸,探针1-A,20个碱基,5' CAL Fluor Red 635,3' Phosphate,双重HPLC纯化:Custom Oligonucleotide, Probe1-A, 20 Bases, 5' CAL Fluor Red 635, 3' Phosphate, Dual HPLC Purified 50 nmol

价格:¥

货号:DNA-1-20BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

https://www.biosearchtech.com/products/custom-oligos/custom-oligos#?tab=product-info 无论您需要标记还是未标记的寡核苷酸或PCR引物,Biosearch Technologies都能满足您的确切需求,因为我们以更具竞争力的价格提供Oligo定制服务。大多数寡核苷酸的合成周期不到5至7个工作日,且可根据需求提供分析证书COA。 LGC Biosearch“构建您的定制寡核苷酸(Build Your Custom Oligos)”服务可定制DNA,RNA,甚至DNA/RNA杂交序列。如果您想订购双标记探针,请使用我们的双标记BHQ®探针快速订购表格。我们还提供其他荧光探针和引物修饰形式。 几个订购小贴士:  1. 如何定制带RNA碱基的寡核苷酸? 通过从Internal Modification(内部修饰)下拉菜单中选择rA,rC,rG或rU,如果您的序列全部是RNA,选中RNA复选框即可。 2. 如何定制具有内部修饰的寡核苷酸? 1)如果您的寡核苷酸需要内部修饰,请选择修饰类型,然后单击添加按钮,内部修饰就会插入到光标的位置。 2)添加由T(...)注释的内部修饰表示一个胸腺嘧啶连接在内部修饰上时,将在序列中引入另外的T碱基。 3. 如何定制具有不确定/降解碱基的寡核苷酸(什么是不确定碱基?请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles) 为了确保寡核苷酸能够被有效识别,当在序列中输入不确定/降解碱基时,请使用IUPAC代码,而不是括号如(A/G),(A/G/C/T)等。 4. 合成规模 vs.更终产量 由于探针/引物在合成过程中需要经过多个步骤,如每个碱基的偶联、寡核苷酸从固相支持物上的切割和纯化步骤,会导致合成规模和更终产量不同。经过这些步骤后,寡核苷酸的更终产率会有损失,将小于合成规模(起始材料)。 5. 订购在板中交付的寡核苷酸 需要订购大量寡核苷酸?请访问我们的高通量定制网页,您可以在其中下载我们的列或行格式excel订单。更少起订量48条。 Whether you require labeled, unlabeled oligos, or pcr primers, Biosearch Technologies can meet your exact needs as we offer custom modifications at extremely competitive rates. Most oligos are shipped in less than five to seven working days. Certificates of Analysis are available upon request. The "Build Your Custom Oligos" Quick Order form allows you to build your custom DNA, RNA, and even DNA/RNA hybrid sequences. If you would like to order a dual-labeled probe, please use our Dual-Labeled BHQ® Probes quick order form. We also offer other fluorogenic probe and primer formats. Don’t see the modification you are looking for, or require a larger synthesis scale? We would be happy advise if we can create your specific oligo. Just ask us at techsupport@biosearchtech.com. A few ordering tips... Building Oligos with RNA Bases Indicate that your are inserting an RNA base by selecting rA, rC, rG or rU from the Internal Modification drop down menu or by checking the RNA checkbox if your sequence is entirely RNA. Building Oligos with Internal Modification(s) 1) If your oligo requires an internal modification, make a selection and then click the ADD button. The internal modification will be inserted at the point of the cursor. 2) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Oligos with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Synthesis Scale vs. Final Yield The synthesis scale and final yield are not the same due to the several steps involved in probe/primer synthesis. These steps include coupling of each base, cleavage of oligonucleotide from solid support and purification steps. The combination of these steps cause the final yield of the oligo to be less than the synthesis scale (starting material). Ordering Oligos Delivered in Plates Ordering a LOT of oligos? Visit our Oligos in Plates webpage where you can download our column or row format excel order forms. Prices start at $0.15 per base with a minimum order of 48 oligos.

定制寡核苷酸,AG_control,26个碱基,5' Thio C6 Linker,3' Methylene Blue,on C7,双重HPLC纯化:Custom Oligonucleotide, AG_control, 26 Bases, 5' Thio C6 Linker, 3' Methylene Blue, on C7, Dual HPLC Purified 1umol

价格:¥

货号:DNA-1-26BASES
品牌:Biosearch

经销:基因生物技术国际贸易(上海)有限公司

https://www.biosearchtech.com/products/custom-oligos/custom-oligos#?tab=product-info 无论您需要标记还是未标记的寡核苷酸或PCR引物,Biosearch Technologies都能满足您的确切需求,因为我们以更具竞争力的价格提供Oligo定制服务。大多数寡核苷酸的合成周期不到5至7个工作日,且可根据需求提供分析证书COA。 LGC Biosearch“构建您的定制寡核苷酸(Build Your Custom Oligos)”服务可定制DNA,RNA,甚至DNA/RNA杂交序列。如果您想订购双标记探针,请使用我们的双标记BHQ®探针快速订购表格。我们还提供其他荧光探针和引物修饰形式。 几个订购小贴士:  1. 如何定制带RNA碱基的寡核苷酸? 通过从Internal Modification(内部修饰)下拉菜单中选择rA,rC,rG或rU,如果您的序列全部是RNA,选中RNA复选框即可。 2. 如何定制具有内部修饰的寡核苷酸? 1)如果您的寡核苷酸需要内部修饰,请选择修饰类型,然后单击添加按钮,内部修饰就会插入到光标的位置。 2)添加由T(...)注释的内部修饰表示一个胸腺嘧啶连接在内部修饰上时,将在序列中引入另外的T碱基。 3. 如何定制具有不确定/降解碱基的寡核苷酸(什么是不确定碱基?请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles) 为了确保寡核苷酸能够被有效识别,当在序列中输入不确定/降解碱基时,请使用IUPAC代码,而不是括号如(A/G),(A/G/C/T)等。 4. 合成规模 vs.更终产量 由于探针/引物在合成过程中需要经过多个步骤,如每个碱基的偶联、寡核苷酸从固相支持物上的切割和纯化步骤,会导致合成规模和更终产量不同。经过这些步骤后,寡核苷酸的更终产率会有损失,将小于合成规模(起始材料)。 5. 订购在板中交付的寡核苷酸 需要订购大量寡核苷酸?请访问我们的高通量定制网页,您可以在其中下载我们的列或行格式excel订单。更少起订量48条。 Whether you require labeled, unlabeled oligos, or pcr primers, Biosearch Technologies can meet your exact needs as we offer custom modifications at extremely competitive rates. Most oligos are shipped in less than five to seven working days. Certificates of Analysis are available upon request. The "Build Your Custom Oligos" Quick Order form allows you to build your custom DNA, RNA, and even DNA/RNA hybrid sequences. If you would like to order a dual-labeled probe, please use our Dual-Labeled BHQ® Probes quick order form. We also offer other fluorogenic probe and primer formats. Don’t see the modification you are looking for, or require a larger synthesis scale? We would be happy advise if we can create your specific oligo. Just ask us at techsupport@biosearchtech.com. A few ordering tips... Building Oligos with RNA Bases Indicate that your are inserting an RNA base by selecting rA, rC, rG or rU from the Internal Modification drop down menu or by checking the RNA checkbox if your sequence is entirely RNA. Building Oligos with Internal Modification(s) 1) If your oligo requires an internal modification, make a selection and then click the ADD button. The internal modification will be inserted at the point of the cursor. 2) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Oligos with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Synthesis Scale vs. Final Yield The synthesis scale and final yield are not the same due to the several steps involved in probe/primer synthesis. These steps include coupling of each base, cleavage of oligonucleotide from solid support and purification steps. The combination of these steps cause the final yield of the oligo to be less than the synthesis scale (starting material). Ordering Oligos Delivered in Plates Ordering a LOT of oligos? Visit our Oligos in Plates webpage where you can download our column or row format excel order forms. Prices start at $0.15 per base with a minimum order of 48 oligos.

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